What are the characteristics of buffer solutions TAE and TBE?

There are roughly three types of electrophoretic buffers used in biochemical research experiments, namely TAE, TBE, and TPE. Although these three buffers have significant effects on DNA separation, TAE and TBE are still commonly used. Because choosing TPE can sometimes lead to a higher concentration of phosphate during the DNA fragment recovery process, making it prone to precipitation, while the other two buffer solutions have lower capacity, are cheaper, and can also achieve better experimental results, many people prioritize TAE and TBE. So what is the difference between the two? Below is a specific introduction for everyone.

1、 TAE buffer

As a widely used buffer, its characteristic is that the measured relative molecular weight is greater than the actual relative molecular weight, and the migration rate of DNA is faster compared to TBE buffer, which can achieve better separation effect. In addition, TAE is also used for electrophoresis during the process of recovering DNA fragments, which is not prone to precipitation. However, when using TAE, it should also be noted that it cannot be subjected to long-term electrophoresis.

2、 TBE buffer, as a commonly used nucleic acid buffer, is mainly used for DNA agarose gel electrophoresis. In addition, it has strong buffer capacity, is suitable for long time electrophoresis detection, and has high resolution, so it may be more widely used than TBE. In addition, because it contains boric acid, which will affect the DNA recovery rate and enzyme reaction, it is not suitable for recovery electrophoresis. If it is used for the agarose gel electrophoresis recovery experiment of DNA fragments, it can be used.

As a professional research and development manufacturer of biological buffer solutions, Desheng mainly produces Tris, Caps, Bicine and other products with a complete range. If special experimental buffer solutions are needed, they can also be customized according to requirements, such as TAE and TBE buffer solutions, which can also be developed and sold. In addition, the buffering agents produced by the company are in powder form to ensure transportation and storage. When used, they need to be prepared into a solution. Based on the preparation method, professional user manuals and dedicated personnel are provided to answer any usage questions. The company provides one-stop solutions to ensure that customers have no worries. If you have any purchasing intentions, please click on the website to inquire about the details!