Poly-L-Lysine(mol wt 150,000)

solid substrates,conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology,microarray glass slide coating,and chromosomal preparations.

Applications for poly-L-lysine include the promotion of cell adhesion to

In order to determine the molecular weight by SEC-LALLS, it is necessary to succinylate the poly-L-lysine.  If this is not done, the  product will form aggregates.

 A method  for molecular weight determination by viscosity has been published.

Purification of high or low molecular weight poly-L-lysines by size exclusion chromatography is not very effective.  Some success has been achieved using succinylated derivatives.  A more traditional method for fractionating polymers, such as dialysis with membranes of suitable molecular weight cut-off, is suggested. 

 None of the poly-L-lysine products are exposed to trifluoroacetic acid (TFA).  They are dialyzed to remove monomer, dimer, trimer, (i.e. short chains) and then analyzed by thin layer- chromatography (TLC) to determine the effectiveness of this process.

Poly-L-lysine is a positively charged amino acid polymer. There is approximately one HBr perlysine residue. The HBr allows the poly-L-lysine to be a crystalline solid and soluble in water.  To remove the HBr, solubilize it in a neutral buffer and dialyze  to  remove the salts.  

To promote the growth of cells.Polylysine adsorption in cell culture, provide large vessels for the surface of cells.