dUTP Chemical Reagents

dUTP may be used instead of dTTP in the PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The Enzyme Uracil-DNA glycosylase, UDG can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives.

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