Molecular Reagents Gel Loding Dye

Gel loading buffer is specially formulated for non-denaturing polyacralamide and agarose gel electrophoresis. The gel loading solution contains bromophenol blue, the tracking dye and sucrose to add density to facilitate sample loading. EDTA has been included to inhibit nucleases that require divalent cations and SDS has also been added to help dissociation of DNA protein complexes, which can interfere in electrophoresis. Bromophenol blue is the most popular tracking dye used in alkaline and neutral buffers systems. Xylene cyanol is another useful tracking dye.