BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit

Specifications of Sheep Anti Interleukin 10 ELISA kit

Product Information

Cat. No. E14A0081

Product Name Sheep Anti Interleukin 10 ELISA kit

Species Sheep

Product Size 48 Tests / 96 Tests

Concentration 25-500 ng/ml

Sensitivity 1.0 ng/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C

Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A (0.5mL) 0 ng/mL 1 vial

STANDARD B (0.5mL) 25 ng/mL 1 vial

STANDARD C (0.5mL) 50 ng/mL 1 vial

STANDARD D (0.5mL) 100 ng/mL 1 vial

STANDARD E (0.5mL) 250 ng/mL 1 vial

STANDARD F (0.5mL) 500 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION (100 x) 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

Anti-il 10 elisa applies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Anti Interleukin 10 ELISA kit

Coefficient of Variance Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery 87-118.37%

Linearity Diluent Ratio Range %

1:2 98-117

1:4 93-106

1:8 91-105

Specificity/Cross-reactivity No significant cross-reactivity or interference between Anti IL 10 and analogues was observed.

Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

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