BlueGene Biotech Bovine Decorin ELISA kit

E11D0145 Bovine Decorin ELISA kit
Bovine Decorin ELISA kit is suitable for the detection of samples from Bovine species. Decorin can also be called CSCD; DSPG2; PG40; PGII; PGS2; PG-S2; SLRR1B; Bone proteoglycan II, DCN.
Specifications of Bovine Decorin ELISA kit
Product Information
Cat. No. E11D0145
Product Name Bovine Decorin ELISA kit
Species Bovine
Product Size 48 Tests / 96 Tests
Concentration 10-250 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8°C
Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 10 ng/mL 1 vial
STANDARD C (0.5mL) 25 ng/mL 1 vial
STANDARD D (0.5mL) 50 ng/mL 1 vial
STANDARD E (0.5mL) 100 ng/mL 1 vial
STANDARD F (0.5mL) 250 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial
Principle of the Assay
DCN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DCN antibody and an DCN-HRP conjugate. The assay sample and buffer are incubated together with DCN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCN concentration since DCN from samples and DCN-HRP conjugate compete for the anti-DCN antibody binding site. Since the number of sites is limited, as more sites are occupied by DCN from the sample, fewer sites are left to bind DCN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCN concentration in each sample is interpolated from this standard curve.
Quality Control on Bovine Decorin ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 90-103%
Linearity Diluent Ratio Range %
1:2 89-105
1:4 86-103
1:8 84-108
Specificity/Cross-reactivity No significant cross-reactivity or interference between DCN and analogues was observed.
Bluegene Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of host cell protein elisa kits for researchers worldwide since 2008.