BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit

E11C0339 Bovine C Type Natriuretic Peptide ELISA kit

Bovine C Type Natriuretic Peptide ELISA kit is suitable for the detection of samples from Bovine species. C Type Natriuretic Peptide can also be called NPPC; CNP2; Natriuretic peptide precursor C; natriuretic peptide C, CTNP.

Specifications of Bovine C Type Natriuretic Peptide ELISA kit

Product Information

Cat. No. E11C0339

Product Name Bovine C Type Natriuretic Peptide ELISA kit

Species Bovine

Product Size 48 Tests / 96 Tests

Concentration 50-1000 pg/mL

Sensitivity 1.0 pg/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C

Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A (0.5mL) 0 pg/mL 1 vial

STANDARD B (0.5mL) 50 pg/mL 1 vial

STANDARD C (0.5mL) 100 pg/mL 1 vial

STANDARD D (0.5mL) 250 pg/mL 1 vial

STANDARD E (0.5mL) 500 pg/mL 1 vial

STANDARD F (0.5mL) 1000 pg/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION (100 x) 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP peptide concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine C Type Natriuretic Peptide ELISA kit

Coefficient of Variance Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery 90-101%

Linearity Diluent Ratio Range %

1:2 87-105

1:4 84-108

1:8 81-108

Specificity/Cross-reactivity No significant cross-reactivity or interference between CNP and analogues was observed.

If you want to know more kinds of anp elisa, please visit our website.

As one bovine igg elisa kits manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGene Biotech ELISA kits.