BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit

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E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit

Bovine Cluster of Differentiation 8 insulin ELISA kit is suitable for the detection of samples from Bovine species. Cluster of Differentiation 8 can also be called P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,CD8.

Specifications of Bovine Cluster Of Differentiation 8 ELISA kit

Product Information

Cat. No. E11C0007

Product Name Bovine Cluster of Differentiation 8 ELISA kit

Species Bovine

Product Size 48 Tests / 96 Tests

Concentration 2.5-50 ng/mL

Sensitivity 0.1 ng/mL

Principal Sandwich ELISA

Sample Volume 50 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C

Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 10 mL 1 vial

STANDARD A (0.5mL) 0 ng/mL 1 vial

STANDARD B (0.5mL) 2.5 ng/mL 1 vial

STANDARD C (0.5mL) 5 ng/mL 1 vial

STANDARD D (0.5mL) 10 ng/mL 1 vial

STANDARD E (0.5mL) 25 ng/mL 1 vial

STANDARD F (0.5mL) 50 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION (100 x) 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD8 antibody and an CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CD8 from samples and CD8-HRP conjugate for the anti-CD8 antibody binding site, the intensity of the color is inversely correlated with the concentration of CD8. As more sites are taken up by CD8 from the sample, fewer sites are left that can bind CD8-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.

Quality Control on Bovine Cluster Of Differentiation 8 ELISA kit

Coefficient of Variance Intra Variation% ＜10%

Inter Variation% ＜12%

Recovery 91-105%

Linearity Diluent Ratio Range %

1:2 88-106

1:4 87-108

1:8 85-110

Specificity/Cross-reactivity No significant cross-reactivity or interference between CD8 and analogues was observed.

Country: Afghanistan
Business Type:
Market:
Founded Year:2007
Address:Lane 166 Tianxiong Road(Rm.403,404, Bldg 2),Pudong NewDistrict,Shanghai,
Contact:Blue gene

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