Introduction of ADPS color reagent
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- China (Mainland)
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- 100
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- 15 Days
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Introduction of ADPS color reagent
In the early years, Trinder reaction was put forward and quickly used. Its main mechanism is that H2O2 produced by the tested substance through enzyme action changes the chromogen substrate
in the presence of 4-AAP and peroxidase. Although phenol will be used as a chromogenic agent at the beginning, with the improvement of reagent, it is found that the reaction sensitivity can be
rapidly improved by using Trinder's new reagent, so it is vigorously promoted and popularized. There are many types of Trinder's reagents, and ADPS is one of them. As a highly water-soluble
aniline derivative, it is widely used in various biochemical kits and diagnostic tests. So we will introduce it in detail.
1、 ADPS Introduction
ADPS, also known as N-ethyl-N - (3-sulfopropyl) - 3-methoxyaniline sodium salt dihydrate, is a common chromogenic substrate for horseradish peroxidase HRP or peroxidase POD. Its appearance is
pure white crystal powder, with purity of more than 99%, PH range of 5.5-9.5, absorption wavelength of 540nm, and obvious advantages of high water solubility and high color sensitivity.
2、 ADPS color rendering principle
The main reason is that under the catalysis of POD, hydrogen peroxide, ADPS and 4AA are coupled and oxidized to produce quinone imines, which will lead to an increase in absorbance at 500nm.
As for blood glucose detection, when blood glucose, oxygen and water are catalysed by POD, they will generate gluconic acid and hydrogen peroxide, and the generated hydrogen peroxide can be
measured by the coupled oxidation system of ADPS. At this time, the degree of absorbance increase is directly proportional to blood glucose. The amount of blood glucose can be measured by measuring
the increase of absorbance with spectrophotometer.
3、 ADPS application
Because of its high sensitivity, safety and stability, ADPS will be used in biochemical kits to detect indicators such as creatinine, glucose, uric acid or cholesterol, further improving the
accuracy of detection results. In addition, at present, major biochemical reagent kit manufacturers or testing instrument companies of peroxidase method are also purchasing and using ADPS in large
quantities, and its application fields are very wide.
Desheng is a manufacturer of new Trinder's reagents. At present, it produces a wide range of products, including ADPS, which can be used in many fields. Of course, it can also be customized
according to its own use. At the same time, the company has its own R&D team, which can provide a series of technical services for follow-up products. If you have relevant requirements, please
click the website or call to inquire details!
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